The Welshman will run the rule over Geoff Cameron, Phil Bardsley and Stephen Ireland ahead of the Potters' first ever visit to the Keepmoat Stadium.
Cameron is nursing an ankle knock suffered in training on Thursday, while Bardsley and Ireland have slight groin and thigh problems respectively.
Hughes will decide upon their availability closer to kick-off at a time where he plans to make a number of changes to the same XI that has previously started each of the last four games.
"Geoff Cameron is the main doubt at the moment," the Potters boss told Stoke City Player.
"He's got an issue with the ligaments in his ankle having gone over on it in training today and at the moment he's probably one who is likely to be missing out at the weekend.
"Phil Bardsley has got an issue with his groin and we'll have to check on him. He's obviously desperate to play so hopefully he is going to be alright and if so it could well be an opportunity for him to play.
"Stephen Ireland meanwhile is a little bit tight too and we'll have to check on him but for the most part we're fine."
Shay Given remains the only definite casualty as he continues on his comeback trail but Hughes is likely to use the fixture to give some much needed playing time to other members of his first team squad.